Preliminary study on cryopreservation of intact mouse ovaries by vitrification using ethylene glycol as cryoprotectant

作者:ZhangJiarong; WanXiaoping; LiShuangdi; LiuLing; YangYixia; YanQin

摘要:Objective: Ultrarapid freezing method (vitrification) is an alternative method for cryopreserving ovarian tissue. The feasibility of intact murine ovaries cryopreservation was studied by vitrification. Methods: Intact mouse ovaries were cryopreserved using vitrification method and slow freezing method. The morphological alterations after frozen-thawed procedure and the apoptosis rates of primordial follicles were evaluated by histological examniation and TUNEL technique, and transmission electron microscopy (TEM) was used to observe the ultrastructural changes. Results:The percentages of normal primordial follicles in frozen-thawed groups were significantly lower than that in the control group ( P<0.001), while no statistical difference was found between vitrification group and slow freezing group (78.37%±4.34% vs 78.82%±3.13%). Moreover, the distribution of abnormalities (nucleus, cytoplasm, and nucleus and cytoplasm) was similar (P>0.05) in these two groups. In the vitrification group, slow freezing group and the fresh control group, the percentages of the TUNEL-positive follicles were 16.37%, 13.29% and 21.36%, respeetively. The apoptosis rates in the two cryopreservation groups showed no significant difference ( P > 0.05). After the freezing-thawing procedure, subeellular structure was well preserved in both freezing groups with smililar ultrastructural changes. Conclusion: Vitrification is a convenient and efficient approach for small ovarian tissue cryopreservation.

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  • 自然科学与工程技术
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  • 医药卫生科技
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  • 妇产科学
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关键词:
  • 形态学
  • 组织学
  • 低温贮藏
  • 电子显微镜
  • 卵巢

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期刊名称:现代妇产科进展

期刊级别:北大期刊

期刊人气:7768

杂志介绍:
主管单位:中华人民共和国教育部
主办单位:山东大学;现代妇产科进展编辑委员会
出版地方:山东
快捷分类:医学
国际刊号:1004-7379
国内刊号:37-1211/R
邮发代号:24-104
创刊时间:1989
发行周期:月刊
期刊开本:A4
下单时间:1-3个月
复合影响因子:1.09
综合影响因子:1.98