The effect of interleukin-6 gene transfer on human cord blood megakaryopoiesis

作者:YangXingsheng; HitoshiKurata; KazuyukiFujita; KenichiTanaka

摘要:Objective:To investigate the effect of IL-6 gene transfer into human cord blood hematopoietic stem cells on the production of megakaryocytic progenitors. Methods: IL-6 gene was transfected into human cord blood CD34 + cells using a retrovirus vector with the aid of recombinant fibronectin fragments in the presence of a cocktail of cytokines (SCF, IL-6, sIL-6R, FL, and TPO). Colony-forming units-megakaryocyte (CFU-MK) assays were perfonned as IL-6 gene transduced CD34 + cells were incubated alone or in combination with IL-3 or sIL-6R, controlled with neoR gene transduced CD34 + cells. Results: IL-6 alone or sIL-6R alone stimulated few CFU-MK colonies, the addition of sIL-6R to IL-6 gene transduced CD34 + cells significantly enhanced the production of CFU-MK colonies. IL-6 gene transduced CD34 + cells showed a modest synergistic effect with IL-3. Conclusion: These results suggest that IL-6 gene transfer may protect patients from chemotherapy-induced thrombocytopenia.

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  • 自然科学与工程技术
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  • 医药卫生科技
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  • 妇产科学
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  • 知网收录(中)
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  • 万方收录(中)
  • 北大期刊(中国人文社会科学期刊)
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  • 统计源期刊(中国科技论文优秀期刊)
关键词:
  • 作用机制
  • 基因转染
  • 造血干细胞
  • 巨核细胞增多症

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期刊名称:现代妇产科进展

期刊级别:北大期刊

期刊人气:7766

杂志介绍:
主管单位:中华人民共和国教育部
主办单位:山东大学;现代妇产科进展编辑委员会
出版地方:山东
快捷分类:医学
国际刊号:1004-7379
国内刊号:37-1211/R
邮发代号:24-104
创刊时间:1989
发行周期:月刊
期刊开本:A4
下单时间:1-3个月
复合影响因子:1.09
综合影响因子:1.98