天然产物研究与开发杂志 北大期刊 CSCD期刊 统计源期刊

天然产物研究与开发 2004年第04期杂志 文档列表

天然产物研究与开发杂志研究论文

根霉3078的代谢产物的研究277-280

摘要：从根霉3078菌丝体的甲醇提取物中分离得到9个化合物,通过波谱分析,鉴定为5α,8α-表二氧-(20S,22E,24R)-麦角甾-6,22-二烯-3β-醇(1)、甘油醇-1-单油酸酯(2)、4-羟基苯乙酮(3)、4-羟基苯乙酸(4)、(20S,22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(5)、(S)-3-羟基-3-苯基丙酸(6)、胸腺嘧啶(7)、尿嘧啶(8)和腺苷(9).

吸附层析分离麻黄生物碱的过程优化281-285

摘要：研究了用吸附层析取代现有的二甲苯萃取麻黄生物碱的工艺,重点考察了洗脱剂和操作条件对产品纯度和回收率的影响,发现在树脂吸附后的洗脱中,0.08 M草酸的洗脱率最高,达99.3%,纯化倍数大于20;在操作条件中,进料量、pH和料液在层析柱中的停留时间影响最大:进料量增大导致纯度和收率的下降,树脂的动态吸附容量为27.5 mg/mL树脂;停留时间在20 min时纯度较高,而洗脱率随停留时间减少却略有下降;pH=10时吸附性能较好.

嗜碱放线菌YIM-80147次生代谢产物的化学成分研究286-289

摘要：对嗜碱放线菌YIM-80147的发酵产物进行化学成分的研究,从中分离得到6个化合物.根据光谱数据的分析,鉴定其结构分别为P371A2(1),4-hydroxymethyl-3-(1-hydroxy-6-methyl-heptyl)-dihydro-furan-2-one(2),nonactic acid(3),homononactinic acid(4),对羟基肉桂酸(5),阿魏酸(6).

野鸦椿酯类化合物抗炎症活性与结构的研究290-293

摘要：从野鸦椿(Euscaphis japoneca Kantiz)枝叶的甲醇提取物中分离得到3个结构相似的酯类化合物,通过波谱分析鉴定为7-hydfoxy-2-octen-5-olide(Euscapholide,1),3,7-dihydfoxy-5-octanolide (2),和methyl 5,7-dihydroxy-2(Z)-octenoate(3).化合物1和3具有较强的抗炎症活性,而结构相似的化合物2却不显示抗炎症活性;通过对这些化合物及诱导体的抗炎症活性的测定,初次确定野鸦椿酯类化合物的抗炎症活性与其结构中的α、β不饱和羰基密切相关,而且有可能是通过抑制体内的环氧酶的活性来实现抗炎症作用的.

藏药裸茎金腰的化学成分研究294-296

摘要：用色谱技术对藏药裸茎金腰(Chrysosplenium nudicaule Bunge)全草化学成分进行分离鉴定,以UV、IR、MS、1H NMR、13C NMR、DEPT等现代谱学方法鉴定化合物结构,得到8个化合物:β巾eltoboykinolic acid,7-O-β-D-glucopyranoside-3′-4′-5-trihydroxy-3,6-dimethoxyflavone,5-甲氧基阿拉伯糖,β-谷甾醇,三十烷,8-甲基-二十八烷,9-甲基-2-三十二烯,1-环戊二烯-3-甲基-十九烷.这8个化合物均为首次从该植物中分离得到.

桂林乌桕中的香豆素和鞣花酸类化合物297-299

摘要：从桂林乌桕枝条的乙醇提取物中分离鉴定了8个已知化合物,经波谱分析确定其结构分别为:6,7,8-三甲氧基香豆素(1)、5,6,7,8-四甲氧基香豆素(2)、8-羟基-5,6,7-三甲氧基香豆素(3)、东莨菪内酯(4)、3,3'-二甲氧基鞣花酸(5)、4,4'-二甲氧基鞣花酸(6)、3,3'-二甲氧基鞣花酸-4-O-βD-木吡喃苷(7)、3,3'-二甲氧基鞣花酸-4-O-β-D-葡萄吡喃苷(8);其中化合物2,3,6,7,8均为首次从该属植物中分离得到.

冬凌草水溶性化学成分研究300-302

摘要：新鲜冬凌革用70%丙酮水溶液组织破碎提取,然后经Diaion HP-20、Toyopearl HW-40、MCI Gel、silicagd等柱层析方法分离得到5个单体化合物,经波谱分析鉴定为α-D-呋喃果糖(1),阿魏酸(2),丹参素甲正丁酯(3),3,4-二羟基苯乳酸(4),延命素-1β-D-吡喃葡萄糖苷(5).除延命素-1β-D-吡喃葡萄糖苷外,其它4个化合物均为首次从冬凌草及其同属植物中分离得到.

Investigation of the flavonoids in Croatian propolis by thin-layer chromatography302-302

摘要：Flavonoids and phenolic acids with a variety of biological activity are considered to be the main compounds in propolis - anatural product produced by the honey bee. TLC can be used for rapid screening of pharmacologically active components and to establish the difference between different propolis samples. Char goal was to optimize chromatographic conditions for separation of flavonoids and phenolic acids and to apply the optimized method for analysis of propolis samples from different geographic regions of Croatia. For chromatographic analysis we used 20 cm x 20 cut glass-backed TLC plates coated with 0.25 mm layers of silica gel 60 F-254.Ethanolic standard solutions (80%) of the flavonoids and phenolic acids (10 muL) were applied to the plates. Chromatograms were developed at room temperature by ascending development in previously saturated vertical, flat-bottomed glass chambers with glass lids. Visualization was performed in short- and long-wavelength UV light and in long-wavelength UV light after spraying with different reagents. After calculation of R F values numerical taxonomy methods were used to test the efficiency of 11 mobile phases and to optimize chromatographic conditions for separation of 19 standard solutions. We established the most appropriate mobile phases (chloroform-methanol-(98-100% ) formic acid, 44.1 + 3 + 2.35, and n-hexane-ethyl acetate-glacial acetic acid, 31 + 14 + 5) for separation of standards. The results obtained were used for analysis of propolis samples. TLC was shown to be a highly suitable method for rapid analysis of propolis samples. It can be used to establish differences between the amounts of pharmacologically active compcunds in propolis from different geographic regions of Croatia.

美丽红豆杉种子化学成分的研究（Ⅰ）303-306

摘要：从美丽红豆杉种子中分离得到1个甾醇和9个紫杉烷类化合物,鉴定为β-谷甾醇(1),5α-cinnamoyloxy-9α,10β,13α-triacetoxy-taxa-4(20),11-diene(2),taxinine(3),taxinine J(4),taxinine B(5),taxinine E(6),10-deacetyltaxinine(7),taxuspineW(8),decinnamoyltaxinine E(9)和紫杉醇(10).除化合物2、3、4以外,其它均为首次从该植物种子中获得.

Synthesis of a malaria candidate glycosylphosphatidylinositol （GPI） structure： A strategy for fully inositol acylated and phosphorylated GPIs306-306

摘要：A congener of the glycosylpbosphatidylinositol (GPI) membrane anchor present on the cell surface of the malaria pathogen Plasmodium, falciparum has been synthesized. This GPI is an example of a small number of such membrane anchors that carry a fatty acyl group at 0-2 of the inositol. Although the acyl group plays crucial roles in GPI biosynthesis, it rarely persits in mature molecules.Other notable examples are the mammalian GPIs CD52 and AchE. The presence of bulky functionalities at three contiguous positions of the inositol moiety creates a very crowded environment that poses difficulties for carrying out selective chemical manipulations.Thus installations of the axial long-chain acyl group and neighboring pbosphoglyceryl complex were fraught with obstacles. The key solution to these obstacles in the successful synthesis of the malarial candidate and prototype structures involved stereoelectronically controlled opening of a cyclic ortho ester. The reaction proceeds in very good yields, the desired axial diastereomer being formed predominantly, even more so in the case of long-chain acyl derivatives. The myoinositol precursor was prepared from methyl alpha-D-glu-copyranoside by the biornimetic procedure of Bender and Budhu. For the glycan array, advantage was taken of the fact that (a) n-pentenyl ortho ester donors are rapidly and chemospecifically activated upon treatment with ytterbium triflate and N-iedosueeinimide and (b) coupling to an aeeeptor affords alpha-coupled product exclusively. A strategy for obtaining the GPI's alpha-glucosaminide component from the corresponding a-mannoside employed Deshong's novel azide displacement procedure. Thus all units of the glycan array were obtained from a beta-D-manno-n-pentenyl ortho ester, this being readily prepared from D-mannose in three easy, high-yielding steps. The "crowded environment" at positions 1 and 2, noted above, could conceivably be relieved by migration of the acyl group to the neighboring cis-O-3-hydroxyl in the natural product. However, study of o

A common sugar as model for many-sided natural product modification. From D-fructose via Dtagatose to 2-C-chlorodifluoro-methylated D-arabinopyranos-5-ulose derivatives306-306

摘要：Starting with 3,4-O- [(R)-2,2,2-trichloroethylidene]-1,2-O-isopropylidene- beta-D- tagatopyranose 2 obtained from 1,2-O-isopropylidene-beta-D-fructopyranose 1 by a non-classical one-step acetalization with chloral/DC~, the fluoroalkylated glyeosyl donors 15 and 17 were synthesised in 3~4 steps. By this sequence, one stereogenic center was inverted, one new chiral center was introduced, and one stereogenic center, for the time being eliminated, was later re-introduced. The glycals 11 and 12, key intermediates of the synthesis sequence, were accessible from triflate precursors (e. g., 10) by treatment with DBU. Corresponding halogeno-(6,7), tosyl-(5, 8), or mesyl-(9) precursors were unsuitable. The stereaselective introduction of a chlorodifluoromethyl group was realised by dithionite-mediated CF2C1Br-addition to the glycal double bond. Subsequently, either the chlorodifluoromethylated glyeosyl bromide (13) or the corresponding pyraneses (14 and 16) were isolated. The latter were still acetylated to the 1-O-acetyl derivatives 15 and 17, respectively. An x-ray analysis is given for the 5-O-tosylate 8.

青蒿鞣质抗病毒活性研究307-311

摘要：从青蒿水提物中分离得到一种缩合鞣质(CTA),在体外进行了抗HSV-2和HBV活性研究.结果表明,CTA具有显著抗HSV-2活性,与阳性对照药物阿昔洛韦(ACV)比较,CC50分别为6.84 mg/mL和3.69mg/mL,IC50分别为0.162 mg/mL和0.138 mg/mL,表明CTA的细胞毒性比ACV小,最低抗病毒活性浓度相当.用含CTA的培养基培养HepG2.2.1.5细胞,结果表明在浓度为2.5 mg/mL时有轻微细胞毒性,在浓度为2.5～0.156 mg/mL对HepG2.2.1.5细胞表达的HBeAg具有显著的抑制作用,0.625 mg/mL浓度档培养第12 d对HBeAg抑制率达到90.45%,提示CTA具有潜在的抗HBV活性.

A New Lariciresinol-Type Butyrolactone Lignan Derived from Hydroxymatairesinol and Its Identification in Spruce Wood311-311

摘要：When the natural lignan hydroxymatairesinol ( 1 ) was treated with an alkaline aqueous solution, it partially rearranged to isomeric forms of a lariciresinol-type butyrolactone lignan. The two major diastereomers formed (2 and 3) were isolated by column and medium-pressure chromatography, and their structures were elucidated by MS and NMR techniques. These previously unknown butyrolactone lignans were identified as naturally occurring in spruce knotwood by GC, GC-MS, and HPLC-ESI MS/MS analyses. The formation of isohydroxymatairesinol (2) and epi-isohydroxymatairesinol (3) from hydroxymatalresinol ( 1 ), and their detection in rat urine after administration of 1, is discussed.

九龙藤化学成分研究（Ⅱ）312-313

摘要：从九龙藤(Bauhinia championii Benth.)的乙醇提取物中分离鉴定5个黄酮类化合物,经波谱分析确定其结构分别为:Quercitrin(1),Myricitrin(2),(-)-Epicatechin gallate(3),5,6,7,3′,4′,5′-hexamethoxyflavone(4),5,6,7,5′-tetramethoxy-3′,4′-methylenedioxyflavone(5).化合物1～3为首次从该植物中分到,另外,本文首次归属了Myricitrin的1H NMR信号.

沙漠绢蒿三萜和酚类化合物的研究314-316

摘要：从沙漠绢蒿(Seriphidium santolium Poliak)全草中首次分离得到7个化合物,通过波谱分析鉴定为蒲公英赛醇(1)、蒲公英赛醇乙酸酯(2)、对羟基反式肉桂酸直链烷基酯类混合物(3)、阿魏酸直链烷基酯类混合物(4)、2-羟基-4,6-二甲氧基乙酰酚酮(5)、4-羟基-2,6-二甲氧基酚-1-O-β-葡萄糖甙(6)、邻-羟基肉桂酸酯甙(7).

采用BSLB（Brine Shrimp Letality Bioassay）法对元宝枫（Acer truncatum Bunge.）及同属植物活性部位的筛选317-319

摘要：本文通过BSLB法对元宝枫及同属植物不同部位的水提物进行了活性试验,得知:元宝枫的幼技、种皮的水提物具一定的生物致死活性;同属植物青榨槭叶和种子,秦岭槭、杈叶槭果翅的水提物也具一定的生物致死活性.本实验为元宝枫及同属植物进行进一步肿瘤细胞的体外培养试验及动物模型试验提供了科学依据.

天然产物研究与开发杂志开发研究

植物源调节剂对玉米生长的影响320-323

摘要：植物源调节剂是利用豆科和菊科植物为原料,首次研制的一种植物生长调节剂,对玉米萌发有促进作用,尤其表现在促进根生长.在苗期、拔节期叶面喷施,提高生物产量21.43%,增加千粒重42.0 g,增产率21.95%.使用浓度为80×10-3g/L或100×10-3g/L使用方法是拌种和苗期、拔节喷施.

天然产物专利信息323-323